5I21
Y55W Hfq from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.860 |
| Spacegroup name | P 6 |
| Unit cell lengths | 114.362, 114.362, 28.343 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.434 - 1.550 |
| R-factor | 0.1889 |
| Rwork | 0.188 |
| R-free | 0.21350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u1s |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.838 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.650 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.046 | 0.522 |
| Number of reflections | 31293 | |
| <I/σ(I)> | 25.89 | 4.09 |
| Completeness [%] | 100.0 | 99 |
| Redundancy | 7.8 | 7.8 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 303 | 7% W/V PEG2000 MME, 2% MPD, 50 MM TRIS-HCL, PH 6.5, |
