5I1L
CRYSTAL STRUCTURE OF HUMAN GERMLINE ANTIBODY IGHV5-51/IGKV4-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-03-26 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.970, 37.960, 112.340 |
| Unit cell angles | 90.00, 100.42, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.950 |
| R-factor | 0.19485 |
| Rwork | 0.194 |
| R-free | 0.25807 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.252 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.060 | 0.395 |
| Number of reflections | 61554 | |
| <I/σ(I)> | 19.7 | 3.1 |
| Completeness [%] | 94.4 | 67.1 |
| Redundancy | 5.2 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 24% PEG 3K, 0.2 M AMMONIUM ACETATE, 0.1 M TRIS PH 8.5 |
