5I1C

CRYSTAL STRUCTURE OF HUMAN GERMLINE ANTIBODY IGHV3-23/IGKV3-20

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	95
Detector technology	CCD
Collection date	2008-10-22
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 62 2 2
Unit cell lengths	121.460, 121.460, 160.390
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.250
R-factor	0.22147
Rwork	0.220
R-free	0.26630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i1d 1dn0
RMSD bond length	0.005
RMSD bond angle	1.055
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.310
High resolution limit [Å]	2.250	2.250
Rmerge	0.086	0.478
Number of reflections	32572
<I/σ(I)>	37	10.4
Completeness [%]	96.9	94.8
Redundancy	27.2	26.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	16% PEG 3K, 0.2 M AMMONIUM ACETATE, 0.1 M MES PH 6.5