5I1A
CRYSTAL STRUCTURE OF HUMAN GERMLINE ANTIBODY IGHV3-23/IGKV3-11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2008-11-11 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.900, 110.550, 158.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| R-factor | 0.20652 |
| Rwork | 0.205 |
| R-free | 0.25533 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5i1c |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.271 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.060 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.066 | 0.204 |
| Number of reflections | 58989 | |
| <I/σ(I)> | 20.4 | 4.6 |
| Completeness [%] | 80.9 | 54.2 |
| Redundancy | 6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 2 M AMMONIUM SULFATE, 5% PEG 400, 0.1 M NA ACETATE PH 4.5 |
