5I19

CRYSTAL STRUCTURE OF HUMAN GERMLINE ANTIBODY IGHV3-23/IGKV1-39

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	95
Detector technology	CCD
Collection date	2009-05-06
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	96.560, 96.560, 105.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.800
R-factor	0.24293
Rwork	0.239
R-free	0.31544
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i1d 4kmt
RMSD bond length	0.009
RMSD bond angle	1.262
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.880
High resolution limit [Å]	2.800	2.800
Rmerge	0.091	0.594
Number of reflections	12678
<I/σ(I)>	31.2	5.1
Completeness [%]	99.5	97.4
Redundancy	14	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 3350, 0.2 M LITHIUM CITRATE