5I15

CRYSTAL STRUCTURE OF HUMAN GERMLINE ANTIBODY IGHV1-69/IGKV1-39

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 31 2 1
Unit cell lengths	129.240, 129.240, 91.840
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.600
R-factor	0.20681
Rwork	0.205
R-free	0.24129
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i18 4kmt
RMSD bond length	0.006
RMSD bond angle	1.191
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.670
High resolution limit [Å]	2.600	2.600
Rmerge	0.048	0.522
Number of reflections	27349
<I/σ(I)>	21.2	3.9
Completeness [%]	99.3	98.7
Redundancy	5	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M MES PH 6.5, 5.0 M SODIUM FORMATE