5I10
Crystal structure of spinosyn rhamnosyl 4'-O-methyltransferase spnh mutant T242Q from Saccharopolyspora Spinosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-28 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 54.776, 54.776, 306.532 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.600 - 2.000 |
| R-factor | 0.2297 |
| Rwork | 0.227 |
| R-free | 0.26370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cdz |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.933 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.600 | 2.150 |
| High resolution limit [Å] | 2.000 | 2.060 |
| Rmerge | 0.054 | 0.880 |
| Number of reflections | 26486 | |
| <I/σ(I)> | 39.04 | 3.23 |
| Completeness [%] | 99.5 | 94.52 |
| Redundancy | 38.4 | 35.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 18-24% (w/v) polyethylene glycol 3350, 100mM Tris, pH 7-9, 200mM magnesium chloride and 2.3mM SRPG |
