5I0Z
Crystal structure of the single domain catalytic antibody 3D8-VH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.24 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 58.775, 85.820, 50.202 |
| Unit cell angles | 90.00, 100.87, 90.00 |
Refinement procedure
| Resolution | 37.402 - 1.900 |
| R-factor | 0.2198 |
| Rwork | 0.218 |
| R-free | 0.26220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gki |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.980 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 16712 | |
| <I/σ(I)> | 6.3 | |
| Completeness [%] | 96.3 | |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 30 % (w/v) PEG 8000 and 0.1 M ammonium sulfate |
