5I0R
D-cysteine bound C93A mutant of Cysteine Dioxygenase at pH 8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2015-03-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.977 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 57.600, 57.600, 122.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.940 - 1.350 |
R-factor | 0.173 |
Rwork | 0.170 |
R-free | 0.20000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4EIO |
RMSD bond length | 0.010 |
RMSD bond angle | 0.973 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((DEV_2386: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.990 | 1.370 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.246 | |
Number of reflections | 46343 | |
<I/σ(I)> | 17.4 | 0.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 53.5 | 36.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1 M tri-sodium citrate pH 5.6, 24% PEG 4000, 0.15 M ammonium acetate |