5I0N
PI4K IIalpha bound to calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9834 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 78.882, 102.028, 78.929 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.950 - 2.280 |
| R-factor | 0.2211 |
| Rwork | 0.219 |
| R-free | 0.25770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pla |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.992 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.950 | 2.360 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.132 | 1.370 |
| Number of reflections | 29715 | |
| <I/σ(I)> | 11.16 | 1.46 |
| Completeness [%] | 99.9 | 99.97 |
| Redundancy | 7.3 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD, 0.1 M bicine/Trizma base pH 8.5 |
