5I0K
Insights into Substrate Modification by Dehydratases from Type I Polyketide Synthases
Replaces: 4P7PExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 83.947, 83.947, 166.982 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.390 - 3.197 |
| R-factor | 0.2545 |
| Rwork | 0.251 |
| R-free | 0.28950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ooc |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.838 |
| Data reduction software | XDS (2.5.3) |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.100 |
| Number of reflections | 10715 |
| <I/σ(I)> | 7.83 |
| Completeness [%] | 95.3 |
| Redundancy | 4.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | Na/K PO4 1.8 M pH 6.0 |
