5I0H
Crystal structure of myosin X motor domain in pre-powerstroke state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 |
| Unit cell lengths | 76.480, 78.300, 78.670 |
| Unit cell angles | 75.69, 86.38, 76.12 |
Refinement procedure
| Resolution | 26.350 - 1.800 |
| R-factor | 0.1666 |
| Rwork | 0.165 |
| R-free | 0.18990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 155150 |
| <I/σ(I)> | 15.96 |
| Completeness [%] | 97.4 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 10% PEG 8000, 50mM Tris pH 7.5, 1mM TCEP and 125mM Lithium sulfate |
