5HZY

Crystal structure of the legionella pneumophila effector protein RavZ - P6322

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-07-14
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9000
Spacegroup name	P 63 2 2
Unit cell lengths	219.272, 219.272, 65.853
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.439 - 2.548
R-factor	0.181
Rwork	0.179
R-free	0.21350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.098
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	41.439
High resolution limit [Å]	2.548
Number of reflections	30876
<I/σ(I)>	47.61
Completeness [%]	100.0
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2.8M Sodium Acetate trihydrate, 200mM NaCl