5HZF
Single Chain Recombinant Globular Head of the Complement System Protein C1q in complex with magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.976 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.996, 52.911, 89.894 |
| Unit cell angles | 90.00, 115.19, 90.00 |
Refinement procedure
| Resolution | 42.901 - 1.550 |
| R-factor | 0.1699 |
| Rwork | 0.169 |
| R-free | 0.18560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pk6 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.188 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 49348 | |
| <I/σ(I)> | 14.85 | 1.95 |
| Completeness [%] | 98.4 | 96.7 |
| Redundancy | 4.9 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 30% PEG 8000, 0.1M HEPES, pH 7.5 |
