5HZD

RNA Editing TUTase 1 from Trypanosoma brucei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-11
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	129.800, 58.130, 66.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.646 - 1.600
R-factor	0.1676
Rwork	0.166
R-free	0.19960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ikf
RMSD bond length	0.010
RMSD bond angle	1.313
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		1.540
High resolution limit [Å]	1.500	6.710	1.500
Rmerge	0.080	0.029	1.644
Number of reflections	81270
<I/σ(I)>	18.72	64.83	1.58
Completeness [%]	99.7	99.5	96.7
Redundancy	13.1
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Tris pH8.5, 19% PEG 3350, 0.2M Lithium sulfate monohydrate