5HZ3

Plant peptide hormone receptor RGFR1 in complex with RGFR5

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-21
Detector	OXFORD RUBY CCD
Wavelength(s)	1
Spacegroup name	H 3
Unit cell lengths	179.174, 179.174, 88.402
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.880 - 2.860
R-factor	0.2431
Rwork	0.243
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	HKL-2000
Phasing software	PHENIX (phenix.refine: 1.8_1069)
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	99.000
High resolution limit [Å]	2.860
Number of reflections	22741
<I/σ(I)>	13.4
Completeness [%]	93.0
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M MES monohydrate pH 6.0, 22% (v/v) polyethylene glycol (PEG) 400.