5HZ2

Crystal structure of PhaC1 from Ralstonia eutropha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-15
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9798
Spacegroup name	I 2 2 2
Unit cell lengths	73.246, 87.856, 136.479
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.800
R-factor	0.1534
Rwork	0.151
R-free	0.18960
Structure solution method	SIRAS
RMSD bond length	0.020
RMSD bond angle	1.952
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.054	0.045	0.261
Number of reflections	40301
<I/σ(I)>	18.7
Completeness [%]	98.1	99.1	93.5
Redundancy	9.8	12.4	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	Ammonium sulfate, HEPES
1	VAPOR DIFFUSION, HANGING DROP	7	295	Ammonium sulfate, HEPES