5HZ2
Crystal structure of PhaC1 from Ralstonia eutropha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-15 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 73.246, 87.856, 136.479 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.1534 |
| Rwork | 0.151 |
| R-free | 0.18960 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.952 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
| Rmerge | 0.054 | 0.045 | 0.261 |
| Number of reflections | 40301 | ||
| <I/σ(I)> | 18.7 | ||
| Completeness [%] | 98.1 | 99.1 | 93.5 |
| Redundancy | 9.8 | 12.4 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | Ammonium sulfate, HEPES |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | Ammonium sulfate, HEPES |
