5HZ1
Plant peptide hormone receptor RGFR1 in complex with RGF3
Experimental procedure
Experimental method | LAUE |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-21 |
Detector | OXFORD RUBY CCD |
Wavelength(s) | 1 |
Spacegroup name | H 3 |
Unit cell lengths | 183.466, 183.466, 87.610 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.420 - 2.590 |
R-factor | 0.2342 |
Rwork | 0.234 |
Structure solution method | MOLECULAR REPLACEMENT |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 99.000 |
High resolution limit [Å] | 2.590 |
Number of reflections | 33780 |
<I/σ(I)> | 31.8 |
Completeness [%] | 98.9 |
Redundancy | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MES monohydrate pH 6.0, 22% (v/v) polyethylene glycol (PEG) 400 |