5HZ0

Plant peptide hormone receptor RGFR1 in complex with RGF2

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-21
Detector	OXFORD SAPPHIRE CCD
Wavelength(s)	1
Spacegroup name	H 3
Unit cell lengths	181.000, 181.000, 87.968
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.940 - 2.560
R-factor	0.2316
Rwork	0.232
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	99.000
High resolution limit [Å]	2.550
Number of reflections	33781
<I/σ(I)>	14.5
Completeness [%]	96.9
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M MES monohydrate pH 6.0, 22%(v/v) polyethylene glycol (PEG) 400.