5HYY

Crystal structure of N-terminal amidase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97951
Spacegroup name	P 42
Unit cell lengths	94.886, 94.886, 61.259
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.509 - 2.323
R-factor	0.1998
Rwork	0.196
R-free	0.24270
RMSD bond length	0.004
RMSD bond angle	0.806
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	37.509
High resolution limit [Å]	2.300
Number of reflections	23487
<I/σ(I)>	42.4
Completeness [%]	99.9
Redundancy	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.2M Ammonium acetate, 0.1M TriNa citrate dihydrate pH5.6, 30%(w/v) PEG 4000