5HYH
CmlI (chemically reduced state), arylamine oxygenase of chloramphenicol biosynthetic pathway
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 56.961, 56.961, 151.099 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.990 - 2.030 |
| R-factor | 0.19308 |
| Rwork | 0.189 |
| R-free | 0.23877 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3chh |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.230 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (v2.5.0) |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.091 | 0.374 |
| Number of reflections | 19182 | |
| <I/σ(I)> | 38 | 5.4 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 6.9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, L-(+)-ammonium tartrate dibasic |
