5HY7
SF3B10-SF3B130 from Chaetomium thermophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 197.083, 197.083, 446.453 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.271 - 2.900 |
| R-factor | 0.2164 |
| Rwork | 0.214 |
| R-free | 0.25320 |
| Structure solution method | AB INITIO PHASING |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.300 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.167 | 3.800 |
| Number of reflections | 113522 | |
| <I/σ(I)> | 19.41 | 0.89 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.1 | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | PEG 4000 8%, Calcium Chloride 0.1 M, Sodium Chloride 0.1 M, HEPES buffer pH 7.5 0.1 M |
