5HY0
orotic acid hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 1 |
| Unit cell lengths | 73.576, 85.661, 87.230 |
| Unit cell angles | 96.47, 114.94, 111.77 |
Refinement procedure
| Resolution | 41.500 - 2.400 |
| R-factor | 0.19644 |
| Rwork | 0.194 |
| R-free | 0.23633 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bvq |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.682 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.300 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 65127 | |
| <I/σ(I)> | 24 | 3.8 |
| Completeness [%] | 97.5 | 83.1 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 281 | Protein was concentrated to 2.1 mg/mL in 100 mM NaCl and 50 mM HEPES pH 7.5; reservoir was 20% PEG 3350, 0.2 M magnesium acetate, 20 mM HEPES pH 6.8 with the Silver Bullets Bio C1 condition of additives; 200 nL of protein added to 90 nL of reservoir and 10 nL of microseeds. |
