5HXT
Crystal Structure of Z,Z-Farnesyl Diphosphate Synthase (D71M, E75A and H103Y Mutants) Complexed with IPP and DMSPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2015-12-24 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.981, 66.321, 128.939 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.150 |
| R-factor | 0.19948 |
| Rwork | 0.197 |
| R-free | 0.24886 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hxn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.185 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.055 | 0.470 |
| Number of reflections | 28515 | |
| <I/σ(I)> | 37.4 | 3.9 |
| Completeness [%] | 99.8 | 98.5 |
| Redundancy | 7.1 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 291 | 13% PEG 10K, 20mM 18-crown-6, 0.1M MES, pH 6.5 |
