5HXQ
Crystal Structure of Z,Z-Farnesyl Diphosphate Synthase (D71M, E75A and H103Y Mutants) Complexed with DMSPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2015-01-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.752, 66.800, 128.726 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.950 |
| R-factor | 0.1964 |
| Rwork | 0.194 |
| R-free | 0.24128 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hxn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.246 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.043 | 0.760 |
| Number of reflections | 38064 | |
| <I/σ(I)> | 42.4 | |
| Completeness [%] | 99.3 | 99.8 |
| Redundancy | 5.9 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 291 | 13% PEG 10K, 20mM 18-crown-6, 0.1M MES, pH 6.5 |
