5HX5

APOBEC3F Catalytic Domain Crystal Structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-31
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	35.940, 50.900, 100.860
Unit cell angles	90.00, 90.75, 90.00

Refinement procedure

Resolution	35.937 - 2.330
R-factor	0.2286
Rwork	0.227
R-free	0.26440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4iou
RMSD bond length	0.008
RMSD bond angle	0.602
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((dev_2313: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.940	2.420
High resolution limit [Å]	2.330	2.330
Rmerge	0.117
Number of reflections	15324
<I/σ(I)>	6.1
Completeness [%]	96.8
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	PEG3350, MgCl2