5HW0

Erwinia chrysanthemi L-asparaginase + Glutamic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-30
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	75.240, 88.818, 174.573
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.763 - 1.702
R-factor	0.1684
Rwork	0.167
R-free	0.20540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1o7j
RMSD bond length	0.006
RMSD bond angle	0.843
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.800
High resolution limit [Å]	1.700	1.700
Rmerge		0.795
Number of reflections	128606
<I/σ(I)>	19.62	2.55
Completeness [%]	99.3	96
Redundancy	7.38	7.2
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	285	24% PEG MME 2000