5HV6
The ATP binding domain of rifampin phosphotransferase from Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 77 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-04-15 |
| Detector | MAR555 FLAT PANEL |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.007, 86.177, 100.304 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.265 - 3.005 |
| R-factor | 0.2463 |
| Rwork | 0.241 |
| R-free | 0.29510 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.028 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 15177 |
| <I/σ(I)> | 37.5 |
| Completeness [%] | 99.9 |
| Redundancy | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 20%(w/v) PEG3350, 200 mM sodium citrate |
