5HUW
Structure of HSV-1 Large Terminase NLS bound to importin alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.260, 90.728, 97.659 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.943 - 1.950 |
| R-factor | 0.1736 |
| Rwork | 0.173 |
| R-free | 0.19460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q5u |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.887 |
| Data reduction software | HKL-2000 (HKL-2000) |
| Data scaling software | HKL-2000 (HKL-2000) |
| Phasing software | PHASER (phaser-MR) |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.784 | |
| Number of reflections | 51772 | |
| <I/σ(I)> | 33.1 | 2.8 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.6M sodium citrate, 100 mM Hepes, pH 6.0, and 10 mM beta-mercaptoethanol |
