5HUG

The crystal structure of neuraminidase from A/American green-winged teal/Washington/195750/2014 influenza virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-26
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 4 21 2
Unit cell lengths	91.644, 91.644, 108.965
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.850
R-factor	0.16598
Rwork	0.164
R-free	0.19504
RMSD bond length	0.020
RMSD bond angle	2.035
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.850
Number of reflections	40498
<I/σ(I)>	27.9
Completeness [%]	99.8
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		293	0.2 M Magnesium Chloride, 0.1 M HEPES:NaOH pH 7.5, 30% (v/v ) PPG P400