5HUF

The crystal structure of hemagglutinin from A/gyrfalcon/Washington/41088-6/2014 influenza virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-06
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	125.328, 252.385, 70.623
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.660 - 2.810
R-factor	0.22943
Rwork	0.228
R-free	0.25627
RMSD bond length	0.012
RMSD bond angle	1.740
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.810
Number of reflections	53667
<I/σ(I)>	12.3
Completeness [%]	96.5
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		293	0.1M Sodium Citrate: Citric acid, pH 5.5, 20% PEG (w/v) 3000