5HUE
DAHP synthase from Corynebacterium glutamicum in complex with tryptophan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 109.189, 109.189, 279.897 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.010 - 2.650 |
| R-factor | 0.24857 |
| Rwork | 0.247 |
| R-free | 0.28463 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b7o |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.257 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.010 | 2.800 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.280 | 7.276 |
| Number of reflections | 29830 | |
| <I/σ(I)> | 9.39 | 0.39 |
| Completeness [%] | 99.7 | 98.8 |
| Redundancy | 17.36 | 17.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 277 | 100 mM NH4 formate, pH 6.6, 100 mM KSCN, 30% PEG 2000 MME |
