5HUC
DAHP synthase from Corynebacterium glutamicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 109.844, 109.844, 279.304 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.450 |
| R-factor | 0.24353 |
| Rwork | 0.243 |
| R-free | 0.25244 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b7o |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.177 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.130 | 2.600 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.099 | 2.950 |
| Number of reflections | 36845 | |
| <I/σ(I)> | 14.02 | 0.64 |
| Completeness [%] | 98.9 | 87.4 |
| Redundancy | 12.86 | 10.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 277 | 100 mM Tris/bicine buffer 13% MPD 13% PEG 1000 13% PEG 3350 2% triethylene glycol 200 uM PEP 100 uM MnCl2 |
