5HT9
Crystal structure of M-crystallin in the presence of nickel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-06-03 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.151, 52.934, 85.933 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.768 - 1.869 |
| R-factor | 0.1697 |
| Rwork | 0.167 |
| R-free | 0.21440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.989 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.4.0) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 1.930 |
| High resolution limit [Å] | 1.860 | 4.000 | 1.860 |
| Rmerge | 0.046 | 0.036 | 0.119 |
| Number of reflections | 12211 | ||
| <I/σ(I)> | 27.5 | ||
| Completeness [%] | 97.4 | 97.6 | 76.7 |
| Redundancy | 4.4 | 4.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 0.2 M Magnesium chloride hexahydrate, 0.1 M BIS-TRIS, 25%(w/v) PEG 3350 |
