5HSW
KSHV SOX RNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 198.200, 45.900, 67.600 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 44.000 - 3.300 |
| R-factor | 0.211 |
| Rwork | 0.208 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pov |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.720 | 3.600 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.140 | 0.700 |
| Rmeas | 0.190 | 0.967 |
| Number of reflections | 9408 | |
| <I/σ(I)> | 6.4 | 1.8 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.993 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.1 M magnesium acetate, 0.1 M MES, pH 6.5, 10% w/v PEG 10,000 |
