5HSO
Crystal structure of MYCOBACTERIUM TUBERCULOSIS MARR FAMILY PROTEIN Rv2887 complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.218, 48.233, 101.115 |
| Unit cell angles | 89.95, 90.04, 107.13 |
Refinement procedure
| Resolution | 36.210 - 2.500 |
| R-factor | 0.2414 |
| Rwork | 0.239 |
| R-free | 0.27760 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.924 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1980) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.210 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.042 | 0.630 |
| Number of reflections | 30122 | |
| <I/σ(I)> | 15.4 | 2 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 6.8 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 0.1 M Potassium chloride, 0.025 M Magnesium chloride hexahydrate, 0.05 M Sodium cacodylate trihydrate pH 6.0, and 15% V/V 2-Propanol |
