5HSM
CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS MARR FAMILY PROTEIN RV2887
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-06-22 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 53.540, 71.290, 72.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.440 - 1.900 |
| R-factor | 0.185 |
| Rwork | 0.184 |
| R-free | 0.20400 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.121 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.440 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.047 | 0.305 |
| Number of reflections | 11335 | |
| <I/σ(I)> | 21.3 | 5.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 289 | 0.1M CITRIC ACID PH 3.5, 2.0 M AMMONIUM SULFATE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 289K |
