5HSJ
Structure of tyrosine decarboxylase complex with PLP at 1.9 Angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97908 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.869, 126.830, 82.877 |
| Unit cell angles | 90.00, 109.73, 90.00 |
Refinement procedure
| Resolution | 49.260 - 1.900 |
| R-factor | 0.17355 |
| Rwork | 0.172 |
| R-free | 0.21243 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hsi |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.129 |
| Phasing software | PHENIX (v1.9) |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 94427 | |
| <I/σ(I)> | 10 | |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 5.2 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 291 | PEG 4000, sodium cacodylate trihydrate, magnesium chloride |
