5HSI
Crystal structure of tyrosine decarboxylase at 1.73 Angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.787, 126.169, 82.728 |
| Unit cell angles | 90.00, 109.89, 90.00 |
Refinement procedure
| Resolution | 25.931 - 1.732 |
| R-factor | 0.1569 |
| Rwork | 0.155 |
| R-free | 0.19050 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.074 |
| Phasing software | PHENIX (v1.9) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.790 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Number of reflections | 115321 | |
| <I/σ(I)> | 20 | |
| Completeness [%] | 93.0 | 89.2 |
| Redundancy | 4.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | PEG1000, magnesium chloride, cacodylate |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | PEG1000, magnesium chloride, cacodylate |
