5HS8
Crystal structure of the diamide-treated YodB from B. subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 62 |
| Unit cell lengths | 95.014, 95.014, 25.434 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.20592 |
| Rwork | 0.204 |
| R-free | 0.24795 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hs7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.459 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.068 | 0.626 |
| Number of reflections | 9187 | |
| <I/σ(I)> | 35.8 | |
| Completeness [%] | 99.9 | |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1M Bis-Tris at pH 5.5, 200mM lithium sulfate, and 25%(w/v) PEG 3,350 |
