5HRI

The crystal structure of AsfvPolX:DNA1 binary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 21 21 2
Unit cell lengths	124.770, 71.273, 86.922
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.360 - 2.200
R-factor	0.20967
Rwork	0.207
R-free	0.25475
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hr9
RMSD bond length	0.006
RMSD bond angle	1.152
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	37862
<I/σ(I)>	38.5
Completeness [%]	99.5
Redundancy	11.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	0.1 M BIS-TRIS pH 5.5, 17% w/v PEG 10000, 0.1 M Ammonium acetate