5HR5
Bovine Heart 6-Phosphofructo-2-Kinase/Fructose-2,6-Bisphosphatase (PFKFB2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CAMD BEAMLINE GCPCC |
Synchrotron site | CAMD |
Beamline | GCPCC |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2014-06-01 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.381 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 82.116, 169.482, 85.254 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.820 |
R-factor | 0.1398 |
Rwork | 0.139 |
R-free | 0.16180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k6m |
RMSD bond length | 0.009 |
RMSD bond angle | 1.099 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((1.10pre_2100)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 46.540 |
High resolution limit [Å] | 1.820 |
Number of reflections | 52151 |
<I/σ(I)> | 2.62 |
Completeness [%] | 97.2 |
Redundancy | 10.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1:1 mixture of protein sample to a mother liquor, pH 7.5 100mM HEPES, pH 7.0 0.5% tacsimate, 13-16% polyethylene glycol 3350, and 3% dioxane. |