5HQJ

Crystal structure of ABC transporter Solute Binding Protein B1G1H7 from Burkholderia graminis C4D1M, target EFI-511179, in complex with D-arabinose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-20
Detector	RAYONIX MX225HE
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	61.094, 37.403, 62.429
Unit cell angles	90.00, 113.61, 90.00

Refinement procedure

Resolution	27.991 - 1.550
R-factor	0.2081
Rwork	0.206
R-free	0.25400
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.355
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MLPHARE
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.610
High resolution limit [Å]	1.550	3.340	1.550
Rmerge	0.057	0.033	0.656
Rmeas	0.061	0.035	0.705
Rpim	0.022	0.013	0.258
Total number of observations	282195
Number of reflections	37889
<I/σ(I)>	10.3
Completeness [%]	99.9	98.7	100
Redundancy	7.4	7.2	7.4
CC(1/2)		0.998	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	Protein (10 mM HEPES pH 7.5, 5 mM DTT, 10 mM D-Arabinose); Reservoir (MCSG2 B2)(0.2 M Potassium Acetate 20 %(w/v) PEG 3350); Cryoprotection (20% Ethylene Glycol, 80% Reservoir)