5HQG

WD40 domain of Human E3 Ubiquitin Ligase COP1 (RFWD2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 31 2 1
Unit cell lengths	68.914, 68.914, 133.922
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.500 - 2.000
R-factor	0.2137
Rwork	0.210
R-free	0.24170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h14
RMSD bond length	0.002
RMSD bond angle	0.573
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.500	2.075
High resolution limit [Å]	2.000	2.000
Rmerge	0.040	0.436
Number of reflections	25511
<I/σ(I)>	20.9	3.29
Completeness [%]	100.0	100
Redundancy	4.9	5
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	sodium nitrate 0.5M 10% PEG 3350