5HPM
Cetuximab Fab in complex with cyclic linked meditope
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-12-20 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.110, 82.760, 212.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.046 - 2.670 |
R-factor | 0.1897 |
Rwork | 0.187 |
R-free | 0.23230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gw1 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.619 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.050 | 2.740 |
High resolution limit [Å] | 2.670 | 2.670 |
Rmerge | 0.218 | |
Number of reflections | 32836 | |
<I/σ(I)> | 8.85 | 1.91 |
Completeness [%] | 99.5 | 100 |
Redundancy | 4.4 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M citrate, 0.1 M sodium phosphate dibasic, 0.5 M potassium phosphate dibasic, 1.6 M sodium phosphate monobasic |