5HPM

Cetuximab Fab in complex with cyclic linked meditope

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-12-20
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	64.110, 82.760, 212.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.046 - 2.670
R-factor	0.1897
Rwork	0.187
R-free	0.23230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gw1
RMSD bond length	0.003
RMSD bond angle	0.619
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.10_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.050	2.740
High resolution limit [Å]	2.670	2.670
Rmerge	0.218
Number of reflections	32836
<I/σ(I)>	8.85	1.91
Completeness [%]	99.5	100
Redundancy	4.4	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M citrate, 0.1 M sodium phosphate dibasic, 0.5 M potassium phosphate dibasic, 1.6 M sodium phosphate monobasic