5HPI
Crystal Structure of the Double Mutant of PobR Transcription Factor Inducer Binding Domain-3-Hydroxy Benzoic Acid complex from Acinetobacter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97934 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 83.490, 125.659, 156.673 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.013 - 2.963 |
| R-factor | 0.1998 |
| Rwork | 0.197 |
| R-free | 0.25790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBID 5HPF |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.977 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10pre_2104: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.157 | 0.827 |
| Number of reflections | 16803 | |
| <I/σ(I)> | 7.49 | 1.3 |
| Completeness [%] | 96.2 | 88.9 |
| Redundancy | 4.3 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 1.0 M Lithium Sulfate, 2.0 % (w/v) PEG 8000 |
