5HP8
Crystal structures of RidA in complex with pyruvate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Agilent SuperNova |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-01-14 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.325, 46.251, 46.375 |
| Unit cell angles | 111.44, 107.74, 106.26 |
Refinement procedure
| Resolution | 22.296 - 2.300 |
| R-factor | 0.2058 |
| Rwork | 0.204 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b33 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.816 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.300 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.065 | 0.276 |
| Number of reflections | 11777 | |
| <I/σ(I)> | 8.2 | 2.5 |
| Completeness [%] | 99.3 | 98.4 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 18% PEG 3350, 0.1M MES pH 6.0 |
