5HP6
Structure of AbnA, a GH43 extracellular arabinanase from Geobacillus stearothermophilus (a new conformational state)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-15 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.847, 82.641, 132.069 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.925 - 2.093 |
| R-factor | 0.1952 |
| Rwork | 0.192 |
| R-free | 0.25250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ho0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.136 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.930 | 2.330 |
| High resolution limit [Å] | 2.093 | 2.093 |
| Rmerge | 0.071 | 0.530 |
| Number of reflections | 45306 | |
| <I/σ(I)> | 23.2 | 3.6 |
| Completeness [%] | 97.6 | 86 |
| Redundancy | 11.34 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 17%-19% PEG 6K, 0.2M NaCl, 0.1M HEPES buffer, pH 7 |
