5HOS
Crystal structure of the endo-beta-1,4-glucanase Xac0029 from Xanthomonas axonopodis pv. citri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-16 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.4587 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 85.604, 85.604, 84.423 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.800 - 1.600 |
| R-factor | 0.1682 |
| Rwork | 0.166 |
| R-free | 0.20380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4w7u |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.306 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.800 | 1.700 | |
| High resolution limit [Å] | 1.600 | 4.780 | 1.600 |
| Rmerge | 0.067 | 0.041 | 0.906 |
| Number of reflections | 47130 | ||
| <I/σ(I)> | 15.14 | 44.36 | 1.28 |
| Completeness [%] | 99.8 | 99.5 | 99.1 |
| Redundancy | 7.06 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25% PEG4000 0.2 M ammonium sulfate 15% glycerol |
