5HOO
Crystal structure of the Mos1 Strand Transfer Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-09 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 256.340, 58.830, 110.140 |
| Unit cell angles | 90.00, 94.91, 90.00 |
Refinement procedure
| Resolution | 60.010 - 3.300 |
| R-factor | 0.2339 |
| Rwork | 0.232 |
| R-free | 0.27390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hos |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.255 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.990 | 3.520 |
| High resolution limit [Å] | 3.290 | 3.290 |
| Number of reflections | 25201 | |
| <I/σ(I)> | 8.1 | 3.3 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 3.1 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | 30% (v/v) MPD, 0.1 M sodium cacodylate pH 6.5,0.2 M magnesium acetate tetrahydrate |
